107021-38-5 | 5-Bromo-4-chloro-3-indolyl-alpha-d-galactopyranoside

Packsize	Purity	Availability	Price	Discounted Price
100mg	98%	in stock	$72.00	$51.00
250mg	98%	in stock	$139.00	$98.00
1g	98%	in stock	$366.00	$257.00
5g	98%	in stock	$1,500.00	$1,050.00

Description

• Catalog Number: AD41105
• Chemical Name: 5-Bromo-4-chloro-3-indolyl-alpha-d-galactopyranoside
• CAS Number: 107021-38-5
• Molecular Formula: C14H15BrClNO6
• Molecular Weight: 408.629
• MDL Number: MFCD00063780
• SMILES: OC[C@H]1O[C@H](Oc2c[nH]c3c2c(Cl)c(cc3)Br)[C@@H]([C@H]([C@H]1O)O)O

Upstream Synthesis Route

X-α-Gal, also known as 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside, is a versatile compound widely used in chemical synthesis applications. This chromogenic substrate is commonly employed in molecular biology and biochemistry research for the detection of β-galactosidase activity.In chemical synthesis, X-α-Gal serves as a substrate for β-galactosidase, an enzyme that cleaves the galactosidic bond within the molecule. Upon hydrolysis by β-galactosidase, X-α-Gal yields a blue product due to the production of a galactose moiety that forms a blue precipitate in the presence of oxygen and a reducing agent. This colorimetric change allows for the visualization and quantification of enzymatic activity in various experimental settings.The unique properties of X-α-Gal make it an indispensable tool for assessing gene expression, screening recombinant plasmids, and monitoring cellular processes. By leveraging the selective enzymatic activity of β-galactosidase, researchers can efficiently track gene expression in bacterial colonies, identify positive clones in cloning experiments, and conduct high-throughput screening assays with ease.Overall, the application of X-α-Gal in chemical synthesis facilitates the rapid and reliable detection of β-galactosidase activity, enabling researchers to gain valuable insights into biological processes and advance their understanding of gene regulation mechanisms.