367-93-1 | (2R,3R,4S,5R,6S)-2-(hydroxymethyl)-6-(propan-2-ylsulfanyl)oxane-3,4,5-triol

Packsize	Purity	Availability	Price	Discounted Price
100mg	98%	in stock	$6.00	$4.00
1g	98%	in stock	$10.00	$7.00
5g	98%	in stock	$15.00	$11.00
25g	95%	in stock	$62.00	$44.00
100g	95%	in stock	$177.00	$124.00
500g	98%	in stock	$713.00	$499.00

Description

• Catalog Number: AI49026
• Chemical Name: (2R,3R,4S,5R,6S)-2-(hydroxymethyl)-6-(propan-2-ylsulfanyl)oxane-3,4,5-triol
• CAS Number: 367-93-1
• Molecular Formula: C9H18O5S
• Molecular Weight: 238.3012
• MDL Number: MFCD00063273
• SMILES: OC[C@H]1O[C@@H](SC(C)C)[C@@H]([C@H]([C@H]1O)O)O

Computed Properties

• Complexity: 201
• Covalently-Bonded Unit Count: 1
• Defined Atom Stereocenter Count: 5
• Heavy Atom Count: 15
• Hydrogen Bond Acceptor Count: 6
• Hydrogen Bond Donor Count: 4
• Rotatable Bond Count: 3
• XLogP3: -0.5

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• A novel family of cyst proteins with epidermal growth factor repeats in Giardia lamblia.

  PLoS neglected tropical diseases 20100501

• [Expression of rat annexin 5 and its effect on human sperm motility in vitro].

  Zhonghua nan ke xue = National journal of andrology 20100501

• Detection of protein-DNA interaction and regulation using gold nanoparticles.

  Analytical biochemistry 20100415

• The E6 protein from vaccinia virus is required for the formation of immature virions.

  Virology 20100410

• [High-level expression and antimicrobial activity of recombinant N-terminal porcine lactoferrin].

  Sheng wu gong cheng xue bao = Chinese journal of biotechnology 20100401

• Enhanced production and characterization of a beta-glucosidase from Bacillus halodurans expressed in Escherichia coli.

  Biochemistry. Biokhimiia 20100401

• A powerful hybrid puc operon promoter tightly regulated by both IPTG and low oxygen level.

  Biochemistry. Biokhimiia 20100401

• [Comparative analysis of nucleotide sequences of lactate dehydrogenase (LDH) gene and LDH epitopes of Plasmodium vivax and Plasmodium falciparum].

  Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases 20100401

• Expression, purification, and characterization of a novel soluble form of human Delta-like-1.

  Applied biochemistry and biotechnology 20100301

• A thermostable phytase from Bacillus sp. MD2: cloning, expression and high-level production in Escherichia coli.

  Journal of industrial microbiology & biotechnology 20100301

• Differential regulation of effector- and central-memory responses to Toxoplasma gondii Infection by IL-12 revealed by tracking of Tgd057-specific CD8+ T cells.

  PLoS pathogens 20100301

• Single-molecule DNA biosensors for protein and ligand detection.

  Angewandte Chemie (International ed. in English) 20100208

• In vitro synthesis of heparosan using recombinant Pasteurella multocida heparosan synthase PmHS2.

  Applied microbiology and biotechnology 20100201

• Anaplasma phagocytophilum Ats-1 is imported into host cell mitochondria and interferes with apoptosis induction.

  PLoS pathogens 20100201

• A new nuclear function of the Entamoeba histolytica glycolytic enzyme enolase: the metabolic regulation of cytosine-5 methyltransferase 2 (Dnmt2) activity.

  PLoS pathogens 20100201

• Cloning, prokaryotic expression, and biological analysis of recombinant chicken IFN-gamma.

  Hybridoma (2005) 20100201

• Gene cloning and catalysis features of a new mannitol-1-phosphate dehydrogenase (BbMPD) from Beauveria bassiana.

  Carbohydrate research 20100111

• Cloning, expression, and characterization of a novel (S)-specific alcohol dehydrogenase from Lactobacillus kefir.

  Applied biochemistry and biotechnology 20100101

• Recombinant human interleukin-24 suppresses gastric carcinoma cell growth in vitro and in vivo.

  Cancer investigation 20100101

• Structural biology of human H3K9 methyltransferases.

  PloS one 20100101

• Expression and purification of recombinant alpha-defensins and alpha-defensin precursors in Escherichia coli.

  Methods in molecular biology (Clifton, N.J.) 20100101

• Dissociation of the octameric enolase from S. pyogenes--one interface stabilizes another.

  PloS one 20100101

• Dynamics of the bacterial intermediate filament crescentin in vitro and in vivo.

  PloS one 20100101

• Negative feedback regulation of Wnt4 signaling by EAF1 and EAF2/U19.

  PloS one 20100101

• Characterization of rubber particles and rubber chain elongation in Taraxacum koksaghyz.

  BMC biochemistry 20100101

• Hypoxia inactivates the VHL tumor suppressor through PIASy-mediated SUMO modification.

  PloS one 20100101

• [Cloning and expression of lipase gene to enantioselective resolution of (S)-ketoprofen].

  Sheng wu gong cheng xue bao = Chinese journal of biotechnology 20100101

• Phosphorylation of SU(VAR)3-9 by the chromosomal kinase JIL-1.

  PloS one 20100101

• Polyclonal antibody against the DPV UL46M protein can be a diagnostic candidate.

  Virology journal 20100101

• Solution structure of the human signaling protein RACK1.

  BMC structural biology 20100101

• Sterol carrier protein-x gene and effects of sterol carrier protein-2 inhibitors on lipid uptake in Manduca sexta.

  BMC physiology 20100101

• Genetic modification of Teredinibacter turnerae, an endosymbiont with biotechnological potential.

  Journal of molecular microbiology and biotechnology 20100101

• Role of type 1 and type 3 fimbriae in Klebsiella pneumoniae biofilm formation.

  BMC microbiology 20100101

• Mathematical modeling: bridging the gap between concept and realization in synthetic biology.

  Journal of biomedicine & biotechnology 20100101

• Insulin degrading enzyme induces a conformational change in varicella-zoster virus gE, and enhances virus infectivity and stability.

  PloS one 20100101

• Identification and characterization of genes associated with tapping panel dryness from Hevea brasiliensis latex using suppression subtractive hybridization.

  BMC plant biology 20100101

• The YEATS family member GAS41 interacts with the general transcription factor TFIIF.

  BMC molecular biology 20100101

• Diversity of protein and mRNA forms of mammalian methionine sulfoxide reductase B1 due to intronization and protein processing.

  PloS one 20100101

• Phage-derived fully human monoclonal antibody fragments to human vascular endothelial growth factor-C block its interaction with VEGF receptor-2 and 3.

  PloS one 20100101

• Enhancement of solubility in Escherichia coli and purification of an aminotransferase from Sphingopyxis sp. MTA144 for deamination of hydrolyzed fumonisin B(1).

  Microbial cell factories 20100101

• Interaction of serum- and glucocorticoid regulated kinase 1 (SGK1) with the WW-domains of Nedd4-2 is required for epithelial sodium channel regulation.

  PloS one 20100101

• Development of infectious cDNA clones of Salmonid alphavirus subtype 3.

  BMC research notes 20100101

• Short-term evaluation in growing rats of diet containing Bacillus thuringiensis Cry1Ia12 entomotoxin: nutritional responses and some safety aspects.

  Journal of biomedicine & biotechnology 20100101

• Detection of sexually transmitted infection and human papillomavirus in negative cytology by multiplex-PCR.

  BMC infectious diseases 20100101

• Mechanical stress activates Smad pathway through PKCδ to enhance interleukin-11 gene transcription in osteoblasts.

  PloS one 20100101

• Lipoprotein biosynthesis as a target for anti-Wolbachia treatment of filarial nematodes.

  Parasites & vectors 20100101

• Designing and engineering evolutionary robust genetic circuits.

  Journal of biological engineering 20100101

• Plasmid DNA delivery into MDA-MB-453 cells mediated by recombinant Her-NLS fusion protein.

  International journal of nanomedicine 20100101

• Recombinant expression screening of P. aeruginosa bacterial inner membrane proteins.

  BMC biotechnology 20100101

• Identification, activity and disulfide connectivity of C-di-GMP regulating proteins in Mycobacterium tuberculosis.

  PloS one 20100101

• Direct injection of functional single-domain antibodies from E. coli into human cells.

  PloS one 20100101

• PICK1 regulates the trafficking of ASIC1a and acidotoxicity in a BAR domain lipid binding-dependent manner.

  Molecular brain 20100101

• Structural and aggregation behavior of the human γD-crystallin mutant E107A, associated with congenital nuclear cataract.

  Molecular vision 20100101

• Structural basis of the association of HIV-1 matrix protein with DNA.

  PloS one 20100101

• Structural basis for the aminoacid composition of proteins from halophilic archea.

  PLoS biology 20091201

• A novel phase variation mechanism in the meningococcus driven by a ligand-responsive repressor and differential spacing of distal promoter elements.

  PLoS pathogens 20091201

• [Expression, purification, and crystallization of a novel galactose mutarotase from Thermoanaerobacter tengcongensis].

  Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae 20091201

• Quantifying pharmacologic suppression of cellular senescence: prevention of cellular hypertrophy versus preservation of proliferative potential.

  Aging 20091201

• [Isolation and identification of a bacterial strain KHg2 with high resistance to mercury and cloning and expression of its merA gene].

  Wei sheng wu xue bao = Acta microbiologica Sinica 20091201

• [Cloning and expression of organic solvent tolerant lipase gene from Staphylococcus saprophyticus M36].

  Sheng wu gong cheng xue bao = Chinese journal of biotechnology 20091201

• Cloning, sequence analysis and expression in E. coli of the group 3 allergen of Dermatophagoides farinae.

  Chinese medical journal 20091105

• Production enhancement and refolding of caprine growth hormone expressed in Escherichia coli.

  Protein expression and purification 20091101

• Optimization of human D-amino acid oxidase expression in Escherichia coli.

  Protein expression and purification 20091101

• cDNA cloning and functional expression of the alpha-D-galactose-binding lectin frutalin in Escherichia coli.

  Molecular biotechnology 20091101

• Development and evaluation of efficient recombinant Escherichia coli strains for the production of 3-hydroxypropionic acid from glycerol.

  Biotechnology and bioengineering 20091101

• Purification and properties of recombinant GST-heparinase III and optimization of cultivation conditions.

  Sheng wu gong cheng xue bao = Chinese journal of biotechnology 20091101

• Optimal production of a novel endo-acting beta-1,4-xylanase cloned from Saccharophagus degradans 2-40 into Escherichia coli BL21(DE3).

  New biotechnology 20091031

• Green tea catechins inhibit the endonuclease activity of influenza A virus RNA polymerase.

  PLoS currents 20091013

• Design and modification of EGF4KDEL 7Mut, a novel bispecific ligand-directed toxin, with decreased immunogenicity and potent anti-mesothelioma activity.

  British journal of cancer 20091006

• Novel strategies to overcome expression problems encountered with toxic proteins: application to the production of Lac repressor proteins for NMR studies.

  Protein expression and purification 20091001

• Redesigning dehalogenase access tunnels as a strategy for degrading an anthropogenic substrate.

  Nature chemical biology 20091001

• Investigation of the interaction between the large and small subunits of potato ADP-glucose pyrophosphorylase.

  PLoS computational biology 20091001

• Analysis of protein expression and a new prokaryotic expression system for goat (Capra hircus) spermadhesin Bdh-2 cDNA.

  Genetics and molecular research : GMR 20090922

• Effect of process parameters on 3-hydroxypropionic acid production from glycerol using a recombinant Escherichia coli.

  Applied microbiology and biotechnology 20090901

• Independently inducible system of gene expression for condensed single protein production (cSPP) suitable for high efficiency isotope enrichment.

  Journal of structural and functional genomics 20090901

• Global analysis of extracytoplasmic stress signaling in Escherichia coli.

  PLoS genetics 20090901

• Enterovirus 71 3C protease cleaves a novel target CstF-64 and inhibits cellular polyadenylation.

  PLoS pathogens 20090901

• High soluble expression of D-amino acid oxidase in Escherichia coli regulated by a native promoter.

  Applied biochemistry and biotechnology 20090801

• Preparation of a biologically active apo-cytochrome b5 via heterologous expression in Escherichia coli.

  Protein expression and purification 20090801

• Production of a cytotoxic cationic antibacterial peptide in Escherichia coli using SUMO fusion partner.

  Applied microbiology and biotechnology 20090801

• Cloning of dextransucrase gene from Leuconostoc citreum HJ-P4 and its high-level expression in E. coli by low temperature induction.

  Journal of microbiology and biotechnology 20090801

• [Prokaryotic expression, purification, and immunogenicity analysis of Mycobacterium tuberculosis specific excretive proteins].

  Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae 20090801

• [Preparation of two antigens--Ag85b and HspX of Mycobacterium tuberculosis H37Rv and the effects of their co-administration with adjuvants in mice].

  Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae 20090801

• [Cloning, expression and characterization of a novel lipase gene lipB from Aspergillus niger F044].

  Wei sheng wu xue bao = Acta microbiologica Sinica 20090801

• Enhancement of surfactin production of Bacillus subtilis fmbR by replacement of the native promoter with the Pspac promoter.

  Canadian journal of microbiology 20090801

• Cloning, high expression and purification of recombinant human intereferon-beta-1b in Escherichia coli.

  Applied biochemistry and biotechnology 20090701

• [Cloning and expression of a gene encoding shortened LfcinB(1-15)-Melittin(5-12) hybrid peptide in Escherichia coli BL21(DE3)].

  Sheng wu gong cheng xue bao = Chinese journal of biotechnology 20090701

• [Prokaryotic expression and polyclonal antibody preparation of TRalphaA in Japanese flounder Paralichthys olivaceus].

  Sheng wu gong cheng xue bao = Chinese journal of biotechnology 20090701

• Rapamycin decelerates cellular senescence.

  Cell cycle (Georgetown, Tex.) 20090615

• Flexibility in the inducer binding region is crucial for allostery in the Escherichia coli lactose repressor.

  Biochemistry 20090609

• Functional expression of Bacillus anthracis protective antigen in E. coli.

  Applied biochemistry and biotechnology 20090601

• An optimized procedure for efficient phage display of antibody fragments with a low folding efficiency.

  Protein expression and purification 20090601

• Purification and characterization of mosquitocidal Bacillus sphaericus BinA protein.

  Journal of invertebrate pathology 20090601

• The pivotal regulator GlnB of Escherichia coli is engaged in subtle and context-dependent control.

  The FEBS journal 20090601

• Direct and indirect roles of His-418 in metal binding and in the activity of beta-galactosidase (E. coli).

  Protein science : a publication of the Protein Society 20090601

• Purification, crystallization and preliminary x-ray crystallographic studies of RAIDD Death-Domain (DD).

  International journal of molecular sciences 20090601

• Induction of extracytoplasmic function sigma factors in Bacillus subtilis cells with membranes of reduced phosphatidylglycerol content.

  Genes & genetic systems 20090601

• Cloning, expression, and purification of Pseudomonas aeruginosa keratinase in Escherichia coli AD494(DE3)pLysS expression system.

  Journal of agricultural and food chemistry 20090513

• Practical protocols for production of very high yields of recombinant proteins using Escherichia coli.

  Protein science : a publication of the Protein Society 20090501

• Investigation of inclusion body formation in recombinant Escherichia coli with a bioimaging system.

  Journal of bioscience and bioengineering 20090501

• Mycobacterium tuberculosis universal stress protein Rv2623 regulates bacillary growth by ATP-Binding: requirement for establishing chronic persistent infection.

  PLoS pathogens 20090501

• [Cloning and characterization of M1 gene of H3N2 subtype swine influenza virus].

  Sheng wu gong cheng xue bao = Chinese journal of biotechnology 20090501

• [Prokaryotic expression and identification of dual-fluorescence fusion proteins of small ubiquitin-like modifier and sentrin-specific protease].

  Sheng wu gong cheng xue bao = Chinese journal of biotechnology 20090501

• [Expression and analysis of the extracellular domain of human glucocorticoid-induced tumor necrosis factor receptor ligand in Escherichia coli].

  Sheng wu gong cheng xue bao = Chinese journal of biotechnology 20090501

• [Study on E protein epitopes and primary identification of main yellow virus].

  Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi 20090501

• Soluble expression in Escherichia coli of active human cyclic nucleotide phosphodiesterase isoform 4B2 in fusion with maltose-binding protein.

  Bioscience, biotechnology, and biochemistry 20090423

• Induction studies with Escherichia coli expressing recombinant interleukin-13 using multi-parameter flow cytometry.

  Biotechnology letters 20090401

• [Correlaion between serum Golph2 protein and hepatocellular carcinoma].

  Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology 20090401

• [Immunoreactivity of the recombinant protein of Echinococcus granulosus antigen B].

  Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases 20090401

• Sus1, Cdc31, and the Sac3 CID region form a conserved interaction platform that promotes nuclear pore association and mRNA export.

  Molecular cell 20090327

• Environmentally-modulated changes in fluorescence distribution in cells with oscillatory genetic network dynamics.

  Journal of biotechnology 20090325

• Expression and purification of Zantedeschia aethiopica agglutinin in Escherichia coli.

  Molecular biology reports 20090301

• High yielding recombinant Staphylokinase in bacterial expression system--cloning, expression, purification and activity studies.

  Protein expression and purification 20090301

• Functional impact of polar and acidic substitutions in the lactose repressor hydrophobic monomer.monomer interface with a buried lysine.

  Biochemistry 20090217

• Enrichment of fermentation media and optimization of expression conditions for the production of EAK(16) peptide as fusions with SUMO.

  Biotechnology and bioengineering 20090215

• Evolving Lac repressor for enhanced inducibility.

  Protein engineering, design & selection : PEDS 20090201

• Determination of viable Escherichia coli using antibody-coated paramagnetic beads with fluorescence detection.

  Analytical and bioanalytical chemistry 20090201

• Expression and purification of bioactive high-purity human midkine in Escherichia coli.

  Journal of Zhejiang University. Science. B 20090201

• The structural basis of gas-responsive transcription by the human nuclear hormone receptor REV-ERBbeta.

  PLoS biology 20090201

• [Recombinant expression and antibacterial activity of i-type lysozyme from sea cucumber Stichopus japonicus].

  Sheng wu gong cheng xue bao = Chinese journal of biotechnology 20090201

• Structure of the pseudokinase VRK3 reveals a degraded catalytic site, a highly conserved kinase fold, and a putative regulatory binding site.

  Structure (London, England : 1993) 20090114

• The diffusive influx and carrier efflux have a strong effect on the bistability of the lac operon in Escherichia coli.

  Journal of theoretical biology 20090107

• E2A acts in cis in G1 phase of cell cycle to promote Ig gene diversification.

  Journal of immunology (Baltimore, Md. : 1950) 20090101

• Characterization of the meningococcal DNA glycosylase Fpg involved in base excision repair.

  BMC microbiology 20090101

• Serum activity of platelet-activating factor acetylhydrolase is a potential clinical marker for leptospirosis pulmonary hemorrhage.

  PloS one 20090101

• Characterisation of immune responses and protective efficacy in mice after immunisation with Rift Valley Fever virus cDNA constructs.

  Virology journal 20090101

• Molecular cloning and characterization of pigeon (Columba liva) ubiquitin and ubiquitin-conjugating enzyme genes from pituitary gland library.

  International journal of biological sciences 20090101

• Mycobacterium tuberculosis Rv3802c encodes a phospholipase/thioesterase and is inhibited by the antimycobacterial agent tetrahydrolipstatin.

  PloS one 20090101

• Export of recombinant proteins in Escherichia coli using ABC transporter with an attached lipase ABC transporter recognition domain (LARD).

  Microbial cell factories 20090101

• Recruitment of HIV-1 envelope occurs subsequent to lipid mixing: a fluorescence microscopic evidence.

  Retrovirology 20090101

• FE65 binds Teashirt, inhibiting expression of the primate-specific caspase-4.

  PloS one 20090101

• Host immune responses to chlamydial inclusion membrane proteins B and C in Chlamydia trachomatis infected women with or without fertility disorders.

  Reproductive biology and endocrinology : RB&E 20090101

• [Construction and expression of the fusion gene CCK39/UreB in recombinant Escherichia coli BL-21(DE3)].

  Sheng wu gong cheng xue bao = Chinese journal of biotechnology 20090101

• RNAi in the cereal weevil Sitophilus spp: systemic gene knockdown in the bacteriome tissue.

  BMC biotechnology 20090101

• The cAMP-HMGA1-RBP4 system: a novel biochemical pathway for modulating glucose homeostasis.

  BMC biology 20090101

• His6-tagged UL35 protein of duck plague virus: expression, purification, and production of polyclonal antibody.

  Intervirology 20090101

• Optimization of a whole-cell cadmium sensor with a toggle gene circuit.

  Biotechnology progress 20090101

• Structural and phylogenetic analysis of a conserved actinobacteria-specific protein (ASP1; SCO1997) from Streptomyces coelicolor.

  BMC structural biology 20090101

• Delineating bacteriostatic and bactericidal targets in mycobacteria using IPTG inducible antisense expression.

  PloS one 20090101

• Production of N-acetyl-D-neuraminic acid using two sequential enzymes overexpressed as double-tagged fusion proteins.

  BMC biotechnology 20090101

• DNA-binding by Haemophilus influenzae and Escherichia coli YbaB, members of a widely-distributed bacterial protein family.

  BMC microbiology 20090101

• Protein kinase Cdelta stimulates proteasome-dependent degradation of C/EBPalpha during apoptosis induction of leukemic cells.

  PloS one 20090101

• Transcriptional effects of CRP* expression in Escherichia coli.

  Journal of biological engineering 20090101

• The germinal center kinase GCK-1 is a negative regulator of MAP kinase activation and apoptosis in the C. elegans germline.

  PloS one 20090101

• Listening to the noise: random fluctuations reveal gene network parameters.

  Molecular systems biology 20090101

• Structural diversity of the active N-terminal kinase domain of p90 ribosomal S6 kinase 2.

  PloS one 20090101

• Stat1 nuclear translocation by nucleolin upon monocyte differentiation.

  PloS one 20090101

• Restoration of contact inhibition in human glioblastoma cell lines after MIF knockdown.

  BMC cancer 20090101

• Sequence Analysis of the Full-length cDNA and Protein Structure Homology Modeling of FABP2 from Paralichthys Olivaceus.

  Bioinformatics and biology insights 20090101

• Identification and sequence determination of recombinant Clostridium perfringens alpha-toxin by use of electrospray ionization mass spectrometry.

  Rinsho Biseibutsu Jinsoku Shindan Kenkyukai shi = JARMAM : Journal of the Association for Rapid Method and Automation in Microbiology 20090101

• Expression, purification and characterisation of recombinant Escherichia coli derived chicken interleukin-12.

  Veterinary immunology and immunopathology 20081215

• Prokaryotic expression, polyclonal antibody preparation of the stilbene synthase gene from grape berry and its different expression in fruit development and under heat acclimation.

  Plant physiology and biochemistry : PPB 20081201

• Influence of N-terminal truncations on the functional expression of Bacillus licheniformis gamma-glutamyltranspeptidase in recombinant Escherichia coli.

  Current microbiology 20081201

• Reconstitution of ThiC in thiamine pyrimidine biosynthesis expands the radical SAM superfamily.

  Nature chemical biology 20081201

• Reliable scale-up of membrane protein over-expression by bacterial auto-induction: from microwell plates to pilot scale fermentations.

  Molecular membrane biology 20081201

• Expression of human peroxisome proliferator-activated receptors ligand binding domain-maltose binding protein fusion protein in Escherichia coli: a convenient and reliable method for preparing receptor for screening ligands.

  Assay and drug development technologies 20081201

• Cloning and expression of antibacterial goat lactoferricin from Escherichia coli AD494(DE3)pLysS expression system.

  Journal of food protection 20081201

• [Co-expression of formate dehydrogenase from Candida boidinii and (R)-specific carbonyl reductase from Candida parapsilosis CCTCC M203011 in Escherichia coli].

  Wei sheng wu xue bao = Acta microbiologica Sinica 20081201

• [Preparation and identification of recombinant maxadilan].

  Sheng wu gong cheng xue bao = Chinese journal of biotechnology 20081201

• Effective high-throughput overproduction of membrane proteins in Escherichia coli.

  Protein expression and purification 20081101

• Cloning, bacterial expression, and unique structure of adenosylhomocysteine hydrolase-like protein 1, or inositol 1,4,5-triphosphate receptor-binding protein from mouse kidney.

  Biochimica et biophysica acta 20081101

• Facile, reagentless and in situ release of Escherichia coli intracellular enzymes by heat-inducible autolytic vector for high-throughput screening.

  Protein engineering, design & selection : PEDS 20081101

• Growth stimulation leads to cellular senescence when the cell cycle is blocked.

  Cell cycle (Georgetown, Tex.) 20081101

• [Cloning and optimizing prokaryotic induced expression conditions of prolactin in White Goose].

  Yi chuan = Hereditas 20081101

• Antimicrobial activity of gamma-thionin-like soybean SE60 in E. coli and tobacco plants.

  Biochemical and biophysical research communications 20081017

• Optimization of glutaryl-7-aminocephalosporanic acid acylase expression in E. coli.

  Protein expression and purification 20081001

• Directing the evolution of Rubisco and Rubisco activase: first impressions of a new tool for photosynthesis research.

  Photosynthesis research 20081001

• Heterologous protein production in Escherichia coli using the propionate-inducible pPro system by conventional and auto-induction methods.

  Protein expression and purification 20081001

• Boron doped diamond biosensor for detection of Escherichia coli.

  Journal of agricultural and food chemistry 20080910

• Characterization and purification of the discoidin domain-containing protein retinoschisin and its interaction with galactose.

  Biochemistry 20080902

• Halogenase genes in nonribosomal peptide synthetase gene clusters of Microcystis (cyanobacteria): sporadic distribution and evolution.

  Molecular biology and evolution 20080901

• Identification of COUP-TFII orphan nuclear receptor as a retinoic acid-activated receptor.

  PLoS biology 20080901

• [Prediction, overexpression and activity confirmation of adenylation domain in Zwittermicin A biosynthesis gene cluster].

  Wei sheng wu xue bao = Acta microbiologica Sinica 20080901

• An erythrocyte vesicle protein exported by the malaria parasite promotes tubovesicular lipid import from the host cell surface.

  PLoS pathogens 20080801

• Influence of signal-peptide truncations on the functional expression of Escherichia coli gamma -glutamyltranspeptidase.

  Journal of basic microbiology 20080801

• [Expression purification and clinical application of high-risk HPV16 E4 gene].

  Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences 20080801

• An in vivo gene deletion system for determining temporal requirement of bacterial virulence factors.

  Proceedings of the National Academy of Sciences of the United States of America 20080708

• Activation of the Escherichia coli marA/soxS/rob regulon in response to transcriptional activator concentration.

  Journal of molecular biology 20080704

• Decrease of 3-hydroxypropionaldehyde accumulation in 1,3-propanediol production by over-expressing dhaT gene in Klebsiella pneumoniae TUAC01.

  Journal of industrial microbiology & biotechnology 20080701

• Intestinal trefoil factor produced in Escherichia coli promotes the healing of rat burn-induced acute gastric mucosal lesions.

  The Journal of trauma 20080701

• [Optimizing expression and purification of recombinant Salvia miltiorrhiza copalyl diphosphate synthase protein in E. coli and preparation of rabbit antiserum against SmCPS].

  Yao xue xue bao = Acta pharmaceutica Sinica 20080701

• [Optimization of prokaryotic expression conditions of Leptospira interrogans trigeminy genus-specific protein antigen based on surface response analysis].

  Wei sheng wu xue bao = Acta microbiologica Sinica 20080701

• [Gene synthesis, expression and immunogenicity analysis of TSP2 hydrophilic domain(TSP2HD) of Schistosoma japonicum].

  Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases 20080630

• Towards toxicity detection using a lab-on-chip based on the integration of MOEMS and whole-cell sensors.

  Biosensors & bioelectronics 20080615

• [Soluble expression, purification and bioactivity of recombinant human Era protein].

  Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology 20080601

• Crystallization and preliminary X-ray diffraction analysis of SeqA bound to a pair of hemimethylated GATC sites.

  Acta crystallographica. Section F, Structural biology and crystallization communications 20080601

• Monitoring of transcriptome and proteome profiles to investigate the cellular response of E. coli towards recombinant protein expression under defined chemostat conditions.

  Journal of biotechnology 20080520

• The metabolic activity of Mycobacterium tuberculosis, assessed by use of a novel inducible GFP expression system, correlates with its capacity to inhibit phagosomal maturation and acidification in human macrophages.

  Molecular microbiology 20080501

• [cDNA cloning and prokaryotic expression of human cholesteryl ester transfer protein and preparation of its antiserum].

  Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology 20080501

• [THz absorption spectra of several carbohydrate derivatives].

  Guang pu xue yu guang pu fen xi = Guang pu 20080501

• [Molecular cloning, recombinant expression and characterization of lysozyme from Chinese shrimp Fenneropenaeus chinensis].

  Sheng wu gong cheng xue bao = Chinese journal of biotechnology 20080501

• Production and characterization of recombinant dengue virus type 4 envelope domain III protein.

  Journal of biotechnology 20080430

• Inactivation of the UGPase1 gene causes genic male sterility and endosperm chalkiness in rice (Oryza sativa L.).

  The Plant journal : for cell and molecular biology 20080401

• Protein production by Escherichia coli wild-type and DeltaptsG mutant strains with IPTG induction at the onset.

  Journal of industrial microbiology & biotechnology 20080401

• Optimization of the production and purification processes of carnobacteriocins Cbn BM1 and Cbn B2 from Carnobacterium maltaromaticum CP5 by heterologous expression in Escherichia coli.

  Journal of microbiological methods 20080401

• Phosphorylation at serines 104 and 106 by Erk1/2 MAPK is important for estrogen receptor-alpha activity.

  Journal of molecular endocrinology 20080401

• Cloning, purification, and polymerization of Capsicum annuum recombinant alpha and beta tubulin.

  Bioscience, biotechnology, and biochemistry 20080401

• [Cloning, prokaryotic expression and functional analysis of pep gene from Bacillus thuringiensis lysogenic bacteriophage].

  Wei sheng wu xue bao = Acta microbiologica Sinica 20080401

• [Construction and culture conditions of dextransucrase-secreting engineered strain].

  Wei sheng wu xue bao = Acta microbiologica Sinica 20080401

• Bioencapsulation of living bacteria (Escherichia coli) with poly(silicate) after transformation with silicatein-alpha gene.

  Biomaterials 20080301

• Identification and functional expression of a Delta9-fatty acid desaturase from Psychrobacter urativorans in Escherichia coli.

  Lipids 20080301

• Cloning and expression of hepatitis C virus core protein in pGemex-1 expression vector.

  Archives of Iranian medicine 20080301

• Expression of soluble, biologically active recombinant human tumstatin in Escherichia coli.

  Clinical and experimental medicine 20080301

• Molecular cloning and characterization of a bile salt hydrolase from Lactobacillus acidophilus PF01.

  Journal of microbiology and biotechnology 20080301

• [Immunoadjuvant effect of the extracellular domain of canine CTLA-4 on the VP2 protein of canine parvovirus].

  Wei sheng wu xue bao = Acta microbiologica Sinica 20080301

• Ligand-induced conformational changes and conformational dynamics in the solution structure of the lactose repressor protein.

  Journal of molecular biology 20080215

• Label-free comparative analysis of proteomics mixtures using chromatographic alignment of high-resolution muLC-MS data.

  Analytical chemistry 20080215

• Preparation of unglycosylated human caseinomacropeptide by engineering DAB Escherichia coli.

  Journal of agricultural and food chemistry 20080213

• Construction of non-toxic Escherichia coli and Vibrio cholerae strains expressing high and immunogenic levels of enterotoxigenic E. coli colonization factor I fimbriae.

  Vaccine 20080206

• Plasmodium falciparum: functional mitochondrial ADP/ATP transporter in Escherichia coli plasmic membrane as a tool for selective drug screening.

  Experimental parasitology 20080201

• Expression of a cytotoxic cationic antibacterial peptide in Escherichia coli using two fusion partners.

  Protein expression and purification 20080201

• Expression and characterization of the acidic subunit from 11S Amaranth seed protein.

  Biotechnology journal 20080201

• Control of acetate production rate in Escherichia coli by regulating expression of single-copy pta using lacI(Q) in multicopy plasmid.

  Journal of microbiology and biotechnology 20080201

• [Expression and purification of human amelogenin in Escherichia coli].

  Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology 20080201

• Functional roles of the hook in a rotating tethered cell.

  Journal of molecular biology 20080111

• Protective effects of recombinant kunitz-domain 1 of human tissue factor pathway inhibitor-2 against 2-chloroethyl ethyl sulfide toxicity in vitro.

  Eplasty 20080101

• UV-A-induced structural and functional changes in human lens deamidated alphaB-crystallin.

  Molecular vision 20080101

• Application of in vivo induced antigen technology (IVIAT) to Bacillus anthracis.

  PloS one 20080101

• Prion protein insertional mutations increase aggregation propensity but not fiber stability.

  BMC biochemistry 20080101

• Xenopus importin beta validates human importin beta as a cell cycle negative regulator.

  BMC cell biology 20080101

• Transduction of E2F-1 TAT fusion proteins represses expression of hTERT in primary ductal breast carcinoma cell lines.

  Molecular cancer 20080101

• Identification and characterization of PhoP regulon members in Yersinia pestis biovar Microtus.

  BMC genomics 20080101

• Human granulocyte colony stimulating factor (hG-CSF): cloning, overexpression, purification and characterization.

  Microbial cell factories 20080101

• Disease mechanism for retinitis pigmentosa (RP11) caused by missense mutations in the splicing factor gene PRPF31.

  Molecular vision 20080101

• A GATA4/WT1 cooperation regulates transcription of genes required for mammalian sex determination and differentiation.

  BMC molecular biology 20080101

• Construction of a series of vectors for high throughput cloning and expression screening of membrane proteins from Mycobacterium tuberculosis.

  BMC biotechnology 20080101

• Autoantigenic nuclear proteins of a clinically atypical renal vasculitis.

  Journal of autoimmune diseases 20080101

• Expression and hydroxylamine cleavage of thymosin alpha 1 concatemer.

  Journal of biomedicine & biotechnology 20080101

• Evolutional selection of a combinatorial phage library displaying randomly-rearranged various single domains of immunoglobulin (Ig)-binding proteins (IBPs) with four kinds of Ig molecules.

  BMC microbiology 20080101

• Development of human antibody fragments using antibody phage display for the detection and diagnosis of Venezuelan equine encephalitis virus (VEEV).

  BMC biotechnology 20080101

• A chemical-genetic strategy reveals distinct temporal requirements for SAD-1 kinase in neuronal polarization and synapse formation.

  Neural development 20080101

• Peptide P5 (residues 628-683), comprising the entire membrane proximal region of HIV-1 gp41 and its calcium-binding site, is a potent inhibitor of HIV-1 infection.

  Retrovirology 20080101

• The POM monoclonals: a comprehensive set of antibodies to non-overlapping prion protein epitopes.

  PloS one 20080101

• Detection of the recombinant proteins in single transgenic microbial cell using laser tweezers and Raman spectroscopy.

  Analytical chemistry 20071215

• Expression of two recombinant chloramphenicol acetyltransferase variants in highly reduced genome Escherichia coli strains.

  Biotechnology and bioengineering 20071201

• Affinity purification of plasmid DNA directly from crude bacterial cell lysates.

  Biotechnology and bioengineering 20071201

• Production of recombinant bacteriocin divercin V41 by high cell density Escherichia coli batch and fed-batch cultures.

  Applied microbiology and biotechnology 20071201

• Prokaryotic expression, purification, and production of polyclonal antibody against human polypeptide N-acetylgalactosaminyltransferase 14.

  Protein expression and purification 20071101

• Genetic method to analyze essential genes of Escherichia coli.

  Applied and environmental microbiology 20071101

• Secretory expression of nattokinase from Bacillus subtilis YF38 in Escherichia coli.

  Molecular biotechnology 20071101

• The role of electrostatics in colicin nuclease domain translocation into bacterial cells.

  The Journal of biological chemistry 20071026

• One step engineering of T7-expression strains for protein production: increasing the host-range of the T7-expression system.

  Protein expression and purification 20071001

• Purification and characterization of two alkaline, thermotolerant alpha-amylases from Bacillus halodurans 38C-2-1 and expression of the cloned gene in Escherichia coli.

  Bioscience, biotechnology, and biochemistry 20071001

• [Highly efficient expression of codon-optimized human papillomavirus 16 L2E7 gene in Escherichia coli].

  Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae 20071001

• Time- and temperature-dependent acetylation of the chemokine RANTES produced in recombinant Escherichia coli.

  Protein expression and purification 20070901

• Cloning, expression, and purification of insecticidal protein Pr596 from locust pathogen Serratia marcescens HR-3.

  Current microbiology 20070901

• The ClosTron: a universal gene knock-out system for the genus Clostridium.

  Journal of microbiological methods 20070901

• Four disulfide-bridged scorpion beta neurotoxin CssII: heterologous expression and proper folding in vitro.

  Biochimica et biophysica acta 20070801

• [Prokaryotic expression, purification and biological activity of recombinant human IL-17/His protein].

  Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology 20070801

• [Prokaryotic expression, purification, refolding and biological assays of recombinant human interleukin 4 inclusion body].

  Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi 20070801

• Enhanced expression and primary purification of soluble HBD3 fusion protein in Escherichia coli.

  Applied biochemistry and biotechnology 20070801

• Cloning and sequence analysis of the gene encoding LipL32 of Leptospira interrogans serovar Sejroe.

  Veterinary research communications 20070701

• Expression and purification of catalytically active human PHD3 in Escherichia coli.

  Protein expression and purification 20070701

• Monitoring dynamic protein expression in living E. coli. Bacterial cells by laser tweezers Raman spectroscopy.

  Cytometry. Part A : the journal of the International Society for Analytical Cytology 20070701

• A tightly regulated and reversibly inducible siRNA expression system for conditional RNAi-mediated gene silencing in mammalian cells.

  The journal of gene medicine 20070701

• Cloning and expression of coxsakievirus B3 viral protein-1 in E. coli.

  Iranian biomedical journal 20070701

• High-level expression of milk-derived antihypertensive peptide in Escherichia coli and its bioactivity.

  Journal of agricultural and food chemistry 20070627

• 5-Aminolevulinate production with recombinant Escherichia coli using a rare codon optimizer host strain.

  Applied microbiology and biotechnology 20070601

• Heterologous expression and characterization of recombinant glycerol dehydratase from Klebsiella pneumoniae in Escherichia coli.

  Biotechnology journal 20070601

• Production of bubaline somatotropin by auto-induction in Escherichia coli.

  Biotechnology and applied biochemistry 20070501

• Over-expression, purification, and characterization of recombinant NAD-malic enzyme from Escherichia coli K12.

  Protein expression and purification 20070501

• Soluble expression of active human beta-defensin-3 in Escherichia coli and its effects on the growth of host cells.

  Chinese medical journal 20070420

• Cloning and expression of the human augmenter of liver regeneration at low temperature in Escherichia coli.

  Journal of biochemical and biophysical methods 20070410

• Construction of recombinant baculovirus Ac-CMV-hSox9 for gene therapy of intervertebral disc degeneration.

  Chinese journal of traumatology = Zhonghua chuang shang za zhi 20070401

• Genetic deletion of a single immunodominant T-cell response confers susceptibility to virus-induced demyelination.

  Brain pathology (Zurich, Switzerland) 20070401

• [Expression of the antibacterial peptide CM4-like gene of Chinese silkworm Bombyx mori in Escherichia coli and its antibacterial activity analysis].

  Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology 20070401

• Tunability and noise dependence in differentiation dynamics.

  Science (New York, N.Y.) 20070323

• Cloning and expression of a human tissue plasminogen activator variant: K2S in Escherichia coli.

  Pakistan journal of biological sciences : PJBS 20070315

• Ligand interactions with lactose repressor protein and the repressor-operator complex: the effects of ionization and oligomerization on binding.

  Biophysical chemistry 20070301

• Large-scale purification and characterization of recombinant Pseudomonas ceramidase: regulation by calcium.

  Journal of lipid research 20070301

• Co-expression of P450 BM3 and glucose dehydrogenase by recombinant Escherichia coli and its application in an NADPH-dependent indigo production system.

  Journal of industrial microbiology & biotechnology 20070301

• Mist1-null mice are resistant to streptozotocin-induced beta cell damage.

  Biochemical and biophysical research communications 20070216

• Expression and purification of a trivalent pertussis toxin-diphtheria toxin-tetanus toxin fusion protein in Escherichia coli.

  Protein expression and purification 20070201

• Heterologous expression and purification of kynurenine-3-monooxygenase from Pseudomonas fluorescens strain 17400.

  Protein expression and purification 20070201

• Production, purification and characterization of recombinant dengue multiepitope protein.

  Biotechnology and applied biochemistry 20070201

• Cell population heterogeneity in expression of a gene-switching network with fluorescent markers of different half-lives.

  Journal of biotechnology 20070201

• Production of recombinant mink growth hormone in E. coli.

  Applied microbiology and biotechnology 20070201

• Tyrosine83 is essential for the activity of E. coli galactoside transacetylase.

  Biochimica et biophysica acta 20070201

• Artificial regulation of ospC expression in Borrelia burgdorferi.

  Molecular microbiology 20070201

• Crystal Structures of the p21-activated kinases PAK4, PAK5, and PAK6 reveal catalytic domain plasticity of active group II PAKs.

  Structure (London, England : 1993) 20070201

• Optimal expression condition of recombinant RAP.

  Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban 20070201

• Improved conditions for production of recombinant plant sesquiterpene synthases in Escherichia coli.

  Protein expression and purification 20070101

• An improved strategy for high-level production of TEV protease in Escherichia coli and its purification and characterization.

  Protein expression and purification 20070101

• One-pot synthesis of genistein from tyrosine by coincubation of genetically engineered Escherichia coli and Saccharomyces cerevisiae cells.

  Applied microbiology and biotechnology 20070101

• Crystal structure of an FIV/HIV chimeric protease complexed with the broad-based inhibitor, TL-3.

  Retrovirology 20070101

• Expression pattern of metallothionein, MTF-1 nuclear translocation, and its dna-binding activity in zebrafish (Danio rerio) induced by zinc and cadmium.

  Environmental toxicology and chemistry 20070101

• Involvement of maternal embryonic leucine zipper kinase (MELK) in mammary carcinogenesis through interaction with Bcl-G, a pro-apoptotic member of the Bcl-2 family.

  Breast cancer research : BCR 20070101

• Ubiquinone-10 production using Agrobacterium tumefaciens dps gene in Escherichia coli by coexpression system.

  Molecular biotechnology 20070101

• An improved high throughput protein-protein interaction assay for nuclear hormone receptors.

  Nuclear receptor signaling 20070101

• Functional expression of an acyl carrier protein (ACP) from Azospirillum brasilense alters fatty acid profiles in Escherichia coli and Brassica juncea.

  Plant physiology and biochemistry : PPB 20070101

• Construction of a high-efficient expression vector of Delta12 fatty acid desaturase in peanut and its prokaryotical expression.

  Journal of genetics and genomics = Yi chuan xue bao 20070101

• Characterization of hypothetical proteins Cpn0146, 0147, 0284 & 0285 that are predicted to be in the Chlamydia pneumoniae inclusion membrane.

  BMC microbiology 20070101

• Functional expression and purification of bovine enterokinase light chain in recombinant Escherichia coli.

  Preparative biochemistry & biotechnology 20070101

• Monitoring of gene knockouts: genome-wide profiling of conditionally essential genes.

  Genome biology 20070101

• Expression, purification, and antibody binding activity of human papillomavirus 16 L1 protein fused to maltose binding protein.

  Protein and peptide letters 20070101

• Interaction of salivary alpha-amylase and amylase-binding-protein A (AbpA) of Streptococcus gordonii with glucosyltransferase of S. gordonii and Streptococcus mutans.

  BMC microbiology 20070101

• Evidence against a blood derived origin for transforming growth factor beta induced protein in corneal disorders caused by mutations in the TGFBI gene.

  Molecular vision 20070101

• Protective effects of recombinant kunitz-domain 1 of human tissue factor pathway inhibitor-2 against 2-chloroethyl ethyl sulfide toxicity in vitro.

  Journal of burns and wounds 20070101

• A global analysis of genetic interactions in Caenorhabditis elegans.

  Journal of biology 20070101

• Signal transducer and activator of transcription 5b: a new target of breast tumor kinase/protein tyrosine kinase 6.

  Breast cancer research : BCR 20070101

• Production of single chain Fab (scFab) fragments in Bacillus megaterium.

  Microbial cell factories 20070101

• Identification of a human immunodominant B-cell epitope within the immunoglobulin A1 protease of Streptococcus pneumoniae.

  BMC microbiology 20070101

• Dynamic rerouting of the carbohydrate flux is key to counteracting oxidative stress.

  Journal of biology 20070101

• Glucose-dependent expansion of pancreatic beta-cells by the protein p8 in vitro and in vivo.

  American journal of physiology. Endocrinology and metabolism 20061201

• Expression of the chalcone synthase gene from grape and preparation of an anti-CHS antibody.

  Protein expression and purification 20061201

• Improving the batch-to-batch reproducibility in microbial cultures during recombinant protein production by guiding the process along a predefined total biomass profile.

  Bioprocess and biosystems engineering 20061201

• ADAM28 participates in the regulation of tooth development.

  Archives of oral biology 20061101

• Optimum melanin production using recombinant Escherichia coli.

  Journal of applied microbiology 20061101

• [Expression, purification, and serum antibody detection of HPV16 E6 in cervical cancer patients].

  Ai zheng = Aizheng = Chinese journal of cancer 20061101

• [The influences of lactose as an inducer on expression of plant des-pGlu1-brazzein in Escherichia coli].

  Sheng wu gong cheng xue bao = Chinese journal of biotechnology 20061101

• Sequential and simultaneous statistical optimization by dynamic design of experiment for peptide overexpression in recombinant Escherichia coli.

  Applied biochemistry and biotechnology 20061001

• Statistical medium formulation and process modeling by mixture design of experiment for peptide overexpression in recombinant Escherichia coli.

  Applied biochemistry and biotechnology 20061001

• [Expression of glutathione-S-transferase fusion protein and human CCL3L1 protein].

  Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae 20061001

• Characterization of the T7 promoter system for expressing penicillin acylase in Escherichia coli.

  Applied microbiology and biotechnology 20060901

• Expression of the cationic antimicrobial peptide lactoferricin fused with the anionic peptide in Escherichia coli.

  Applied microbiology and biotechnology 20060901

• A link in transcription between the native pbpB and the acquired mecA gene in a strain of Staphylococcus aureus.

  Microbiology (Reading, England) 20060901

• Expression, purification and anti-tumor activity of curcin.

  Acta biochimica et biophysica Sinica 20060901

• [Preparation and identification of polyclonal antiserum against heat shock protein 72].

  Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue 20060901

• Tracking the in vivo fate of recombinant polypeptides by isotopic labeling.

  Journal of controlled release : official journal of the Controlled Release Society 20060828

• Isolation of a cDNA encoding a protease from Perinereis aibuhitensis Grube.

  Acta biochimica et biophysica Sinica 20060801

• Simple affinity chromatographic procedure to purify beta-galactoside binding lectins.

  Journal of chromatography. B, Analytical technologies in the biomedical and life sciences 20060711

• Size characterization of green fluorescent protein inclusion bodies in E. coli using asymmetrical flow field-flow fractionation-multi-angle light scattering.

  Journal of chromatography. A 20060707

• Detection of gentoxicity of benzidine and its derivatives with the Escherichia coli DJ 702 lacZ reversion mutagenicity assay.

  Letters in applied microbiology 20060701

• Cloning and expression of an alpha-1,3-glucanase gene from Bacillus circulans KA-304: the enzyme participates in protoplast formation of Schizophyllum commune.

  Bioscience, biotechnology, and biochemistry 20060701

• The protective immune response induced by B cell epitope of classical swine fever virus glycoprotein E2.

  Journal of virological methods 20060601

• Bacterial fermentation of recombinant major wasp allergen Antigen 5 using oxygen limiting growth conditions improves yield and quality of inclusion bodies.

  Protein expression and purification 20060601

• The galactophilic lectin, LecA, contributes to biofilm development in Pseudomonas aeruginosa.

  Environmental microbiology 20060601

• Recombinant human B7-H4 expressed in Escherichia coli inhibits T lymphocyte proliferation and IL-2 secretion in vitro.

  Acta pharmacologica Sinica 20060601

• Optimization and high-level expression of a functional GST-tagged rHLT-B in Escherichia coli and GM1 binding ability of purified rHLT-B.

  Journal of microbiology (Seoul, Korea) 20060601

• Design of a peptibody consisting of the antimicrobial peptide dhvar5 and a llama variable heavy-chain antibody fragment.

  Chemical biology & drug design 20060601

• [Prokaryotic expression and purification of recombination Salmonella invasion protein].

  Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition 20060501

• [Purification of cadmium ion binding metallothionein-3 by proteinase digestion on affinity chromatographic column].

  Se pu = Chinese journal of chromatography 20060501

• Expression and purification of a fusion-typed pediocin PA-1 in Escherichia coli and recovery of biologically active pediocin PA-1.

  International journal of food microbiology 20060415

• The GTPase, CpgA(YloQ), a putative translation factor, is implicated in morphogenesis in Bacillus subtilis.

  Molecular genetics and genomics : MGG 20060401

• Purification, crystallization and preliminary X-ray diffraction studies of N-acetylglucosamine-phosphate mutase from Candida albicans.

  Acta crystallographica. Section F, Structural biology and crystallization communications 20060401

• [Production of Antibody against Helicobacter pylori HP0231.].

  The Korean journal of laboratory medicine 20060401

• Dominant-negative Rac1 suppresses Ras-induced apoptosis possibly through activation of NFkappaB in Ha-ras oncogene-transformed NIH/3T3 cells.

  Life sciences 20060313

• A recombinant vaccine against hydatidosis: production of the antigen in Escherichia coli.

  Journal of industrial microbiology & biotechnology 20060301

• A bottom-up approach to gene regulation.

  Nature 20060216

• Expression and purification of Arisaema heterophyllum agglutinin in Escherichia coli.

  Journal of plant physiology 20060201

• Mapping structural interactions using in-cell NMR spectroscopy (STINT-NMR).

  Nature methods 20060201

• Cloning and expression of Mycobacterium bovis secreted protein MPB83 in Escherichia coli.

  Journal of biochemistry and molecular biology 20060131

• Identification of anthranilate and benzoate metabolic operons of Pseudomonas fluorescens and functional characterization of their promoter regions.

  Microbial cell factories 20060101

• The inflammatory and normal transcriptome of mouse bladder detrusor and mucosa.

  BMC physiology 20060101

• Direct interaction between the catalytic subunit of Protein Phosphatase 1 and pRb.

  Cancer cell international 20060101

• The DtxR protein acting as dual transcriptional regulator directs a global regulatory network involved in iron metabolism of Corynebacterium glutamicum.

  BMC genomics 20060101

• Comparative analysis of Saccharomyces cerevisiae WW domains and their interacting proteins.

  Genome biology 20060101

• Construction of a nurse shark (Ginglymostoma cirratum) bacterial artificial chromosome (BAC) library and a preliminary genome survey.

  BMC genomics 20060101

• Effect of inducers on the production of 5-aminolevulinic acid by recombinant Escherichia coli.

  Preparative biochemistry & biotechnology 20060101

• Reversible inactivation of the transcriptional function of P53 protein by farnesylation.

  BMC biotechnology 20060101

• Epigenetic acquisition of inducibility of type III cytotoxicity in P. aeruginosa.

  BMC bioinformatics 20060101

• Arabinose-induction of lac-derived promoter systems for penicillin acylase production in Escherichia coli.

  Biotechnology progress 20060101

• Maximization of production of his-tagged glycine oxidase and its M261 mutant proteins.

  Biotechnology progress 20060101

• Co-expression of chaperonin GroEL/GroES enhances in vivo folding of yeast mitochondrial aconitase and alters the growth characteristics of Escherichia coli.

  The international journal of biochemistry & cell biology 20060101

• Molecular and immunological characterization of allergens from the entomopathogenic fungus Beauveria bassiana.

  Clinical and molecular allergy : CMA 20060101

• A novel assay for monitoring internalization of nanocarrier coupled antibodies.

  BMC immunology 20060101

• Regulating expression of pyruvate kinase in Bacillus subtilis for control of growth rate and formation of acidic byproducts.

  Biotechnology progress 20060101

• The eggshell is required for meiotic fidelity, polar-body extrusion and polarization of the C. elegans embryo.

  BMC biology 20060101

• Structure of vaccinia virus thymidine kinase in complex with dTTP: insights for drug design.

  BMC structural biology 20060101

• Effects of the presence of ColE1 plasmid DNA in Escherichia coli on the host cell metabolism.

  Microbial cell factories 20060101

• In-a-day electrochemical detection of coliforms in drinking water using a tyrosinase composite biosensor.

  Analytical chemistry 20051215

• Involvement of polyubiquitin chains via specific chain linkages in stress response in mammalian cells.

  Biological & pharmaceutical bulletin 20051201

• [Construction of fusion expression vector of human-derived neurotrophin-6 gene encoding mature peptide and purification of its expressed product].

  Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi 20051201

• An improved method for the purification of rat liver-type fatty acid binding protein from Escherichia coli.

  Protein expression and purification 20051101

• The effects of ftsZ mutation on the production of recombinant protein in Bacillus subtilis.

  Applied microbiology and biotechnology 20051101

• Construction of plasmid-based expression vectors for Bacillus subtilis exhibiting full structural stability.

  Plasmid 20051101

• Monitoring 'promiscuous' drug effects on single cells of multiple cell types.

  Analytical biochemistry 20051015

• Evidence that entry into sporulation in Bacillus subtilis is governed by a gradual increase in the level and activity of the master regulator Spo0A.

  Genes & development 20050915

• Integrated insights from simulation, experiment, and mutational analysis yield new details of LacI function.

  Biochemistry 20050823

• Optimization of extracellular production of recombinant asparaginase in Escherichia coli in shake-flask and bioreactor.

  Applied microbiology and biotechnology 20050801

• Production and characterisation of gilthead sea bream (Sparus auratus) recombinant parathyroid hormone related protein.

  General and comparative endocrinology 20050801

• [Establishment of human endothelial-overexpressed lipopolysaccharide-associated factor 1 compelling expression model and its effects on the proliferation of ECV304 cells].

  Zhonghua shao shang za zhi = Zhonghua shaoshang zazhi = Chinese journal of burns 20050801

• Development of a latex agglutination test using the major epitope domain of glycoprotein E of pseudorabies virus expressed in E. coli to differentiate between immune responses in pigs naturally infected or vaccinated with pseudorabies virus.

  Veterinary research communications 20050801

• Influence of induction and operation mode on recombinant rhamnulose 1-phosphate aldolase production by Escherichia coli using the T5 promoter.

  Journal of biotechnology 20050721

• A peptide triggers allostery in tet repressor by binding to a unique site.

  The Journal of biological chemistry 20050701

• The Lac repressor provides a reversible gene expression system in undifferentiated and differentiated embryonic stem cell.

  Cellular and molecular life sciences : CMLS 20050701

• Long-term monitoring of bacteria undergoing programmed population control in a microchemostat.

  Science (New York, N.Y.) 20050701

• Application of the 'codon-shuffling' method. Synthesis and selection of de novo proteins as antibacterials.

  The Journal of biological chemistry 20050624

• Protein production by auto-induction in high density shaking cultures.

  Protein expression and purification 20050501

• Effect of postinduction nutrient feed composition and use of lactose as inducer during production of thermostable xylanase in Escherichia coli glucose-limited fed-batch cultivations.

  Journal of bioscience and bioengineering 20050501

• Expression, mutagenesis, and characterization of recombinant low-potential cytochrome c550 of photosystem II.

  Biochemistry 20050426

• Solubility and proteolysis of the Zb-MalE and Zb-MalE31 proteins during overproduction in Escherichia coli.

  Biotechnology and bioengineering 20050420

• Transcriptome profiles for high-cell-density recombinant and wild-type Escherichia coli.

  Biotechnology and bioengineering 20050420

• Sensitivity analysis of discrete stochastic systems.

  Biophysical journal 20050401

• Secretory expression of thermostable T1 lipase through bacteriocin release protein.

  Protein expression and purification 20050401

• Group I metabotropic glutamate receptors, mGlu1a and mGlu5a, couple to cyclic AMP response element binding protein (CREB) through a common Ca2+ - and protein kinase C-dependent pathway.

  Journal of neurochemistry 20050401

• Radiation abolishes inducer binding to lactose repressor.

  Radiation research 20050401

• Induction of protective immunity against scrub typhus with a 56-kilodalton recombinant antigen fused with a 47-kilodalton antigen of Orientia tsutsugamushi Karp.

  The American journal of tropical medicine and hygiene 20050401

• Noise propagation in gene networks.

  Science (New York, N.Y.) 20050325

• A novel 'DEAD-box' DNA helicase from Plasmodium falciparum is homologous to p68.

  Molecular and biochemical parasitology 20050301

• Yersinia pestis outer membrane type III secretion protein YscC: expression, purification, characterization, and induction of specific antiserum.

  Protein expression and purification 20050301

• Production of recombinant amyloid-beta peptide 42 as an ubiquitin extension.

  Protein expression and purification 20050301

• Binding of estrogenic compounds to recombinant estrogen receptor-alpha: application to environmental analysis.

  Environmental health perspectives 20050301

• [Cloning and expression of superoxide dismutase gene from Deinococcus radiodurans in E. coli].

  Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition 20050301

• [Purification and identification of recombinant varicella-zoster virus glycoprotein E].

  Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition 20050301

• Expression of lexA targeted ribozyme in Escherichia coli BL-21 (DE3) cells.

  Molecular and cellular biochemistry 20050301

• Determination of thio-based additives for biopharmaceuticals by pulsed electrochemical detection following HPLC.

  Journal of pharmaceutical and biomedical analysis 20050207

• Expression, refolding, purification, and bioactivity of recombinant bifunctional protein, hIL-2/GM-CSF.

  Protein expression and purification 20050201

• Optimization of expression and purification of two biologically active chimeric fusion proteins that consist of human interleukin-13 and Pseudomonas exotoxin in Escherichia coli.

  Protein expression and purification 20050201

• Expression and purification of histidine-tagged bacteriophage T7 DNA polymerase.

  Protein expression and purification 20050201

• Heterologous expression of hexaheme fragments of a multidomain cytochrome from Geobacter sulfurreducens representing a novel class of cytochromes c.

  Protein expression and purification 20050201

• Expression, purification of human vasostatin120-180 in Escherichia coli, and its anti-angiogenic characterization.

  Protein expression and purification 20050201

• Selective cadmium accumulation using recombinant Escherichia coli.

  Journal of bioscience and bioengineering 20050201

• Expression pattern of epithelial cell adhesion molecule on normal and malignant colon tissues.

  World journal of gastroenterology 20050121

• The use of transformed Escherichia coli for experimental angiogenesis induced by regulated in situ production of vascular endothelial growth factor--an alternative gene therapy.

  Medical hypotheses 20050101

• Cloning, expression, purification, and analysis of mannitol dehydrogenase gene mtlK from Lactobacillus brevis.

  Applied biochemistry and biotechnology 20050101

• [A preliminary study on the expression and biological function of recombinant human SCF-TPO fusion protein].

  Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi 20050101

• Effects of culturing conditions on production of D-hydantoinase from recombinant Escherichia coli.

  Journal of microbiology, immunology, and infection = Wei mian yu gan ran za zhi 20041201

• [Co-expression of beta-subunit with other subunits of Qbeta replicase].

  Wei sheng wu xue bao = Acta microbiologica Sinica 20041201

• Expression, purification, and characterization of His-tagged penicillin G acylase from Kluyvera citrophila in Escherichia coli.

  Protein expression and purification 20041101

• Coexpression, purification and characterization of the E and S subunits of coenzyme B(12) and B(6) dependent Clostridium sticklandii D-ornithine aminomutase in Escherichia coli.

  European journal of biochemistry 20041101

• Schistosoma japonicum: construction of phage display antibody library and its application in the immunodiagnosis of infection.

  Chinese medical journal 20041101

• [Effects of lactose inducing on expression of Helicobacter pylori rUreB and rHpaA, and Escherichia coli rLTKA63 and rLTB].

  Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences 20041101

• [Construction and expression of plasmids containing different truncated core proteins of hepatitis C virus derived from tumor and non-tumor tissues].

  Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology 20041101

• Expression and purification of a novel rice (Oryza sativa L.) mitochondrial ATP synthase small subunit in Escherichia coli.

  Protein expression and purification 20041001

• Cloning, expression, and purification of the virulence-associated protein D from Xylella fastidiosa.

  Protein expression and purification 20041001

• Intein-mediated fusion expression, high efficient refolding, and one-step purification of gelonin toxin.

  Protein expression and purification 20041001

• Optimised expression and purification of recombinant human indoleamine 2,3-dioxygenase.

  Protein expression and purification 20041001

• Construction of prokaryotic expression system of ltB-ureB fusion gene and identification of the recombinant protein immunity and adjuvanticity.

  World journal of gastroenterology 20040915

• Arachidonic acid-induced carbon-centered radicals and phospholipid peroxidation in cyclo-oxygenase-2-transfected PC12 cells.

  Journal of neurochemistry 20040901

• Using networks to identify fine structural differences between functionally distinct protein states.

  Biochemistry 20040831

• Tightly regulated vectors for the cloning and expression of toxic genes.

  Journal of microbiological methods 20040801

• Oncogenic Ras dominates overexpression of E-cadherin in malignant transformation of intestinal epithelial cells.

  Surgery 20040801

• Cloning, sequencing and expression of GroEL-like protein gene of Vibrio parahaemolyticus.

  Journal of microbiology, immunology, and infection = Wei mian yu gan ran za zhi 20040801

• Contribution of Müller cells toward the regulation of photoreceptor outer segment assembly.

  Neuron glia biology 20040801

• Vectors for regulated gene expression in the radioresistant bacterium Deinococcus radiodurans.

  Gene 20040707

• Induction of transcription by p21Waf1/Cip1/Sdi1: role of NFkappaB and effect of non-steroidal anti-inflammatory drugs.

  Cell cycle (Georgetown, Tex.) 20040701

• Inhibition of [(15)N]valine transamination during selective labeling of barstar in a T7 polymerase system.

  Biochemistry. Biokhimiia 20040701

• Mirk/dyrk1B kinase destabilizes cyclin D1 by phosphorylation at threonine 288.

  The Journal of biological chemistry 20040625

• Effects of lactose as an inducer on expression of Helicobacter pylori rUreB and rHpaA, and Escherichia coli rLTKA63 and rLTB.

  World journal of gastroenterology 20040615

• Reversible modulation of cell cycle kinetics in NIH/3T3 mouse fibroblasts by inducible overexpression of mitochondrial manganese superoxide dismutase.

  Antioxidants & redox signaling 20040601

• Human recombinant B7-H3 expressed in E. coli enhances T lymphocyte proliferation and IL-10 secretion in vitro.

  Acta biochimica et biophysica Sinica 20040601

• Growth inhibition of Escherichia coli during heterologous expression of Bacillus subtilis glutamyl-tRNA synthetase that catalyzes the formation of mischarged glutamyl-tRNA1 Gln.

  Journal of microbiology (Seoul, Korea) 20040601

• Limited role of N-terminal phosphoserine residues in the activation of transcription by p53.

  Oncogene 20040527

• Conditional gene silencing utilizing the lac repressor reveals a role of SHP-2 in cagA-positive Helicobacter pylori pathogenicity.

  Cancer science 20040501

• Bacteria co-transformed with recombinant proteins and chaperones cloned in independent plasmids are suitable for expression tuning.

  Journal of biotechnology 20040408

• Synechocystis Fe superoxide dismutase gene confers oxidative stress tolerance to Escherichia coli.

  Biochemical and biophysical research communications 20040402

• sll1722, an unassigned open reading frame of Synechocystis PCC 6803, codes for L-myo-inositol 1-phosphate synthase.

  Planta 20040401

• Expression and purification of recombinant cytoplasmic domain of human erythrocyte band 3 with hexahistidine tag or chitin-binding tag in Escherichia coli.

  Protein expression and purification 20040401

• Functional expression and display of an antibody Fab fragment in Escherichia coli: study of vector designs and culture conditions.

  Protein expression and purification 20040401

• Construction of prokaryotic expression system of ureB gene from a clinical Helicobacter pylori strain and identification of the recombinant protein immunity.

  World journal of gastroenterology 20040401

• Heterologous expression of a gene encoding a 35 kDa protein of Mycobacterium avium paratuberculosis in Escherichia coli.

  Veterinary research communications 20040401

• Cloning and expression in Escherichia coli of the D-aspartate oxidase gene from the yeast Cryptococcus humicola and characterization of the recombinant enzyme.

  Journal of biochemistry 20040401

• High-level expression and purification of untagged and histidine-tagged HIV-1 reverse transcriptase.

  Protein expression and purification 20040301

• Enhanced expression of a recombinant malaria candidate vaccine in Escherichia coli by codon optimization.

  Protein expression and purification 20040301

• [Study on the reconstitution in vitro and photochemical activities of phytochrome from the Synechocystis sp. PCC6803].

  Sheng wu gong cheng xue bao = Chinese journal of biotechnology 20040301

• Platelet-activating factor-induced apoptosis is blocked by Bcl-2 in rat intestinal epithelial cells.

  American journal of physiology. Gastrointestinal and liver physiology 20040201

• Cloning and GST-fused expression in E. coli of mouse beta-1,4-galactosyltransferase.

  Journal of Zhejiang University. Science 20040201

• Production of 3-hydroxydecanoic acid by recombinant Escherichia coli HB101 harboring phaG gene.

  Antonie van Leeuwenhoek 20040201

• Antigenicity and receptor-binding ability of recombinant SARS coronavirus spike protein.

  Biochemical and biophysical research communications 20040123

• Expression and purification of the recombinant SALT lectin from rice (Oryza sativa L.).

  Protein expression and purification 20040101

• Roles of phosphatidylinositol 3'-kinase and mammalian target of rapamycin/p70 ribosomal protein S6 kinase in K-Ras-mediated transformation of intestinal epithelial cells.

  Cancer research 20040101

• Evaluation of recombinant green fluorescent protein, under various culture conditions and purification with HiTrap hydrophobic interaction chromatography resins.

  Applied biochemistry and biotechnology 20040101

• Visualization of the dynamics of gene expression in the living mouse.

  Molecular imaging 20040101

• Copy-control tightly regulated expression vectors based on pBAC/oriV.

  Methods in molecular biology (Clifton, N.J.) 20040101

• A catalog of human cDNA expression clones and its application to structural genomics.

  Genome biology 20040101

• Branched-chain amino acid aminotransferase and methionine formation in Mycobacterium tuberculosis.

  BMC microbiology 20040101

• Chromosomal duplications and cointegrates generated by the bacteriophage lamdba Red system in Escherichia coli K-12.

  BMC molecular biology 20040101

• Expression and purification of SARS coronavirus membrane protein.

  Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban 20040101

• Recombinant alkaline serine protease II degrades scrapie isoform of prion protein.

  In vitro cellular & developmental biology. Animal 20040101

• Insights into the relation between mrna and protein expression patterns: II. Experimental observations in Escherichia coli.

  Biotechnology and bioengineering 20031230

• Permissive glycan support of photoreceptor outer segment assembly occurs via a non-metabolic mechanism.

  Molecular vision 20031216

• Use of the transport specificity ratio and cysteine-scanning mutagenesis to detect multiple substrate specificity determinants in the consensus amphipathic region of the Escherichia coli GABA (gamma-aminobutyric acid) transporter encoded by gabP.

  The Biochemical journal 20031215

• Perturbation from a distance: mutations that alter LacI function through long-range effects.

  Biochemistry 20031202

• Optimization of recombinant aminolevulinate synthase production in Escherichia coli using factorial design.

  Applied microbiology and biotechnology 20031201

• [Vector construction for the intracellular domain of human receptor for advanced glycation end- products and expression of the fusion protein].

  Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA 20031201

• Recombinant CD64-specific single chain immunotoxin exhibits specific cytotoxicity against acute myeloid leukemia cells.

  Cancer research 20031201

• Construction of high-density display of CD147 ectodomain on VCSM13 phage via gpVIII: effects of temperature, IPTG, and helper phage infection-period.

  Protein expression and purification 20031201

• Structural basis for the altered activity of Gly794 variants of Escherichia coli beta-galactosidase.

  Biochemistry 20031125

• Development of a fed-batch fermentation process to overproduce phosphoenolpyruvate carboxykinase using an expression vector with promoter and plasmid copy number controllable by heat.

  Biotechnology and bioengineering 20031120

• The expression of spinach glycolate oxidase (GO) in E. coli and the application of GO in the production of glyoxylic acid.

  Molecular biotechnology 20031101

• Differences in properties between human alphaA- and alphaB-crystallin proteins expressed in Escherichia coli cells in response to cold and extreme pH.

  The Biochemical journal 20031015

• Construction of expression systems for flaA and flaB genes of Helicobacter pylori and determination of immunoreactivity and antigenicity of recombinant proteins.

  World journal of gastroenterology 20031015

• Retinoblastoma protein (pRB) was significantly phosphorylated through a Ras-to-MAPK pathway in mutant K-ras stably transfected human adrenocortical cells.

  DNA and cell biology 20031001

• Expression and purification of receptor for activated C-kinase 1 (RACK1).

  Protein expression and purification 20030901

• Reduced sulfhydryls maintain specific incision of BPDE-DNA adducts by recombinant thermoresistant Bacillus caldotenax UvrABC endonuclease.

  Protein expression and purification 20030901

• Purification of recombinant plasminogen activator inhibitor-1 in the active conformation by refolding from inclusion bodies.

  Protein expression and purification 20030901

• Expression, purification, and characterization of human enteropeptidase catalytic subunit in Escherichia coli.

  Protein expression and purification 20030901

• Bacterial expression, purification, and characterization of rat kidney-type mitochondrial glutaminase.

  Protein expression and purification 20030901

• Dialysis strategies for protein refolding: preparative streptavidin production.

  Protein expression and purification 20030901

• Purification of recombinant human apometallothionein-3 and reconstitution with zinc.

  Protein expression and purification 20030901

• Galactose induces the expression of penicillin acylase under control of the lac promoter in recombinant Escherichia coli.

  Biotechnology letters 20030901

• [Expression of recombinant human BMP-6 in Escherichia coli and its purification and bioassay in vitro].

  Sheng wu gong cheng xue bao = Chinese journal of biotechnology 20030901

• Direct detection and quantification of horizontal gene transfer by using flow cytometry and gfp as a reporter gene.

  Current microbiology 20030801

• Antisense-mediated loss of calcium homoeostasis endoplasmic reticulum protein (CHERP; ERPROT213-21) impairs Ca2+ mobilization, nuclear factor of activated T-cells (NFAT) activation and cell proliferation in Jurkat T-lymphocytes.

  The Biochemical journal 20030701

• Extremely low frequency magnetic fields affect transposition activity in Escherichia coli.

  Radiation and environmental biophysics 20030701

• Construction and expression of a humanized M2 autoantigen trimer and its application in the diagnosis of primary biliary cirrhosis.

  World journal of gastroenterology 20030615

• Production of Bacillus anthracis protective antigen is dependent on the extracellular chaperone, PrsA.

  The Journal of biological chemistry 20030516

• Synthesis of isobutyl-C-galactoside (IBCG) as an isopropylthiogalactoside (IPTG) substitute for increased induction of protein expression.

  Organic letters 20030515

• Cooperation between RNA polymerase molecules in transcription elongation.

  Science (New York, N.Y.) 20030502

• LacSwitch II regulation of connexin43 cDNA expression enables gap-junction single-channel analysis.

  BioTechniques 20030501

• cDNA cloning of growth hormone from giant panda (Ailuropoda melanoleuca) and its expression in Escherichia coli.

  Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology 20030501

• Preparation of humanized ovarian carcinoma anti-idiotypic minibody.

  Hybridoma and hybridomics 20030401

• Purification of human alpha-L-fucosidase precursor expressed in Escherichia coli as a glutathione S-transferase fusion protein.

  Journal of chromatography. B, Analytical technologies in the biomedical and life sciences 20030325

• Glutathione depletion-induced apoptosis of Ha-ras-transformed NIH3T3 cells can be prevented by melatonin.

  Oncogene 20030306

• Characterization of a novel inhibitory feedback of the anti-anti-sigma SpoIIAA on Spo0A activation during development in Bacillus subtilis.

  Molecular microbiology 20030301

• Expression and purification of invasion plasmid antigen C of Shigella flexneri.

  Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA 20030301

• The 5-enolpyruvylshikimate-3-phosphate synthase of glyphosate-tolerant soybean expressed in Escherichia coli shows no severe allergenicity.

  Molecules and cells 20030227

• Enhanced oligomerization of the alpha-synuclein mutant by the Cu,Zn-superoxide dismutase and hydrogen peroxide system.

  Molecules and cells 20030227

• Expression of the serum- and glucocorticoid-inducible protein kinase, Sgk, is a cell survival response to multiple types of environmental stress stimuli in mammary epithelial cells.

  The Journal of biological chemistry 20030221

• Construction and characterization of a chimeric myoglobin.

  Biochimica et biophysica acta 20030221

• Generation and characterization of recombinant single chain Fv antibody that recognizes platelet glycoprotein Ibalpha.

  Thrombosis research 20030125

• A stereodivergent, two-directional synthesis of stereoisomeric C-linked disaccharide mimetics.

  Organic & biomolecular chemistry 20030121

• Role of a highly conserved bacterial protein in outer membrane protein assembly.

  Science (New York, N.Y.) 20030110

• Applicability of new expression vectors for both engineering uses and biological studies.

  Biotechnology progress 20030101

• Type III secretion proteins PcrV and PcrG from Pseudomonas aeruginosa form a 1:1 complex through high affinity interactions.

  BMC microbiology 20030101

• [Cloning and expression of the signaling protein 14-3-3 of Toxoplasma gondii].

  Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases 20030101

• Large-scale production of genetically engineered CYP3A4 in E. coli: application of a jarfermenter.

  Drug metabolism and pharmacokinetics 20030101

• A new member of the GM130 golgin subfamily is expressed in the optic lobe anlagen of the metamorphosing brain of Manduca sexta.

  Journal of insect science (Online) 20030101

• Of mice and models: improved animal models for biomedical research.

  Physiological genomics 20021203

• Overexpression of monkey oviductal protein: purification and characterization of recombinant protein and its antibodies.

  Biology of reproduction 20021201

• Production of heterologous thermostable glycoside hydrolases and the presence of host-cell proteases in substrate limited fed-batch cultures of Escherichia coli BL21(DE3).

  Applied microbiology and biotechnology 20021201

• Internal response correction for fluorescent whole-cell biosensors.

  Analytical chemistry 20021201

• Production of extracellular domain of human tissue factor using maltose-binding protein fusion system.

  Protein expression and purification 20021101

• Mutant K-ras oncogene regulates steroidogenesis of normal human adrenocortical cells by the RAF-MEK-MAPK pathway.

  British journal of cancer 20021021

• Allelic variants of the human glutathione S-transferase P1 gene confer differential cytoprotection against anticancer agents in Escherichia coli.

  Pharmacogenetics 20021001

• [Cloning, purification and biological activity of human vascular endothelial growth factor fragment in E. coli].

  Zhonghua zhong liu za zhi [Chinese journal of oncology] 20020901

• Genetic networks. Small numbers of big molecules.

  Science (New York, N.Y.) 20020816

• Stochastic gene expression in a single cell.

  Science (New York, N.Y.) 20020816

• Production and characterisation of a recombinant scFv reactive with human gastrointestinal carcinomas.

  British journal of cancer 20020812

• Expression and one-step purification of a developmentally regulated protein from Dictyostelium discoideum.

  Protein expression and purification 20020801

• Purification and characterization of the fusion protein trypsin-streptavidin expressed in Escherichia coli.

  Journal of protein chemistry 20020801

• Development of an inducible system to assess p94 (CAPN3) function in cultured muscle cells.

  Journal of biotechnology 20020703

• Herpes simplex virus type 2 US3 blocks apoptosis induced by sorbitol treatment.

  Microbes and infection 20020601

• Expression and purification of the recombinant enteropathogenic Escherichia coli vaccine candidates BfpA and EspB.

  Protein expression and purification 20020601

• Expression, purification and characterization of a recombinant levan fructotransferase.

  Biotechnology and applied biochemistry 20020601

• Expression of Escherichia coli glutaredoxin 2 is mainly regulated by ppGpp and sigmaS.

  The Journal of biological chemistry 20020517

• Evidence that the putative alpha-glucosidase of Thermotoga maritima MSB8 is a pNP alpha-D-glucuronopyranoside hydrolyzing alpha-glucuronidase.

  FEBS letters 20020424

• H2O2 induces a transient multi-phase cell cycle arrest in mouse fibroblasts through modulating cyclin D and p21Cip1 expression.

  The Journal of biological chemistry 20020419

• Molecular characterization of a specific thiamine triphosphatase widely expressed in mammalian tissues.

  The Journal of biological chemistry 20020419

• Structure of the lac operon galactoside acetyltransferase.

  Structure (London, England : 1993) 20020401

• Hsp72 expression enhances survival in adenosine triphosphate-depleted renal epithelial cells.

  Cell stress & chaperones 20020401

• Quantitative proteomics strategy involving the selection of peptides containing both cysteine and histidine from tryptic digests of cell lysates.

  Journal of chromatography. A 20020308

• Expression of ayu (Plecoglossus altivelis) Pit-1 in Escherichia coli: its purification and immunohistochemical detection using monoclonal antibody.

  Protein expression and purification 20020301

• C/EBPalpha triggers proteasome-dependent degradation of cdk4 during growth arrest.

  The EMBO journal 20020301

• Overexpression of a recombinant wild-type and His-tagged Bacillus subtilis glycine oxidase in Escherichia coli.

  European journal of biochemistry 20020301

• High production of heterologous proteins in Escherichia coli using the thermo-regulated T7 expression system.

  Applied microbiology and biotechnology 20020301

• Isolation and characterization of cbbL and cbbS genes encoding form I ribulose-1,5-bisphosphate carboxylase/oxygenase large and small subunits in Nitrosomonas sp. strain ENI-11.

  Bioscience, biotechnology, and biochemistry 20020301

• [Construction of the recombinant plasmid FasAD-pTYB2 capable of Fas activation domain fusion expression].

  Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA 20020301

• High-level expression of human extracellular superoxide dismutase in Escherichia coli and insect cells.

  Protein expression and purification 20020201

• Functional regions of rice heat shock protein, Oshsp16.9, required for conferring thermotolerance in Escherichia coli.

  Plant physiology 20020201

• Consequences of RNase E scarcity in Escherichia coli.

  Molecular microbiology 20020201

• A carboxylesterase from the hyperthermophilic archaeon Sulfolobus solfataricus: cloning of the gene, characterization of the protein.

  Gene 20020123

• Role of pRB-family/E2F complex in the inhibition of IL-3-dependent lymphoid cell proliferation.

  Cytokine 20020121

• An in vivo screening system against protein splicing useful for the isolation of non-splicing mutants or inhibitors of the RecA intein of Mycobacterium tuberculosis.

  Gene 20020109

• Towards genetic genome projects: genomic library screening and gene-targeting vector construction in a single step.

  Nature genetics 20020101

• Expression of alpha subunit of Gs in Escherichia coli.

  Methods in enzymology 20020101

• Periplasmic expression and purification of recombinant Fabs.

  Methods in molecular biology (Clifton, N.J.) 20020101

• [Cloning and expression of Buthus martensii Karsch scorpion toxin gene (BmK IT3) in Escherichia coli].

  Sheng wu gong cheng xue bao = Chinese journal of biotechnology 20020101

• Hydrolysis of lactose by beta-glycosidase CelB from hyperthermophilic archaeon Pyrococcus furiosus: comparison of hollow-fiber membrane and packed-bed immobilized enzyme reactors for continuous processing of ultrahigh temperature-treated skim milk.

  Applied biochemistry and biotechnology 20020101

• Effect of lacY expression on homogeneity of induction from the P(tac) and P(trc) promoters by natural and synthetic inducers.

  Biotechnology progress 20020101

• [Cloning and expression of spinach glycolate oxidase in Escherichia coli].

  Sheng wu gong cheng xue bao = Chinese journal of biotechnology 20020101

• [Construction of an artificial digenic network with epigenetic properties].

  Molekuliarnaia biologiia 20020101

• Pyridoxal 5'-phosphate inhibits DNA binding of HNF1.

  Biochimica et biophysica acta 20011219

• Human angiogenin fused to human CD30 ligand (Ang-CD30L) exhibits specific cytotoxicity against CD30-positive lymphoma.

  Cancer research 20011215

• Overexpression, purification, and characterization of glutaminase-interacting protein, a PDZ-domain protein from human brain.

  Protein expression and purification 20011201

• Isopropyl-beta-D-thiogalactosidase (IPTG)-inducible tyrosine phosphorylation of proteins in E. coli.

  BioTechniques 20011201

• An improved procedure for production of human epidermal growth factor from recombinant E. coli.

  Applied microbiology and biotechnology 20011201

• Rapid orientated cloning in a shuttle vector allowing modulated gene expression in Bacillus subtilis.

  FEMS microbiology letters 20011127

• [The role of p21 (wafl/cip1) in human colorectal carcinoma cell apoptosis].

  Zhonghua yi xue za zhi 20011110

• Synthesis of a hexasaccharide that relates to the arabinogalactan epitope.

  Carbohydrate research 20011108

• High-level expression and single-step purification of human tryptophanyl-tRNA synthetase.

  Protein expression and purification 20011101

• Secretory production of human granulocyte colony-stimulating factor in Escherichia coli.

  Protein expression and purification 20011101

• An in vivo study of novel bioactive peptides that inhibit the growth of Escherichia coli.

  The journal of peptide research : official journal of the American Peptide Society 20011101

• Expression of an active recombinant lysine 49 phospholipase A(2) myotoxin as a fusion protein in bacteria.

  Toxicon : official journal of the International Society on Toxinology 20011001

• High resolution structural analyses of mutant chitinase A complexes with substrates provide new insight into the mechanism of catalysis.

  Biochemistry 20010925

• Protein kinase Calpha expression confers retinoic acid sensitivity on MDA-MB-231 human breast cancer cells.

  Experimental cell research 20010910

• The N-terminal signal sequence and the last 98 amino acids are not essential for the secretion of Bacillus sp. TS-23 alpha-amylase in Escherichia coli.

  Current microbiology 20010901

• Regulation of phospholipase C-gamma(1) by the actin-regulatory protein villin.

  American journal of physiology. Cell physiology 20010901

• Expression of soluble form carp (Cyprinus carpio) ovarian cystatin in Escherichia coli and its purification.

  Journal of agricultural and food chemistry 20010901

• CP43', the isiA gene product, functions as an excitation energy dissipator in the cyanobacterium Synechococcus sp. PCC 7942.

  Photochemistry and photobiology 20010901

• [The bottleneck steps limiting maturation of penicillin G acylase in Escherichia coli].

  Sheng wu gong cheng xue bao = Chinese journal of biotechnology 20010901

• An experimental and theoretical study of the inhibition of Escherichia coli lac operon gene expression by antigene oligonucleotides.

  Biotechnology and bioengineering 20010805

• Oncogenic ras represses transforming growth factor-beta /Smad signaling by degrading tumor suppressor Smad4.

  The Journal of biological chemistry 20010803

• Cloning and overexpression in soluble form of functional shikimate kinase and 5-enolpyruvylshikimate 3-phosphate synthase enzymes from Mycobacterium tuberculosis.

  Protein expression and purification 20010801

• A new method for protein coexpression in Escherichia coli using two incompatible plasmids.

  Protein expression and purification 20010801

• Expression of active human C1 inhibitor serpin domain in Escherichia coli.

  Protein expression and purification 20010701

• Changing colors in mice: an inducible system that delivers.

  Genes & development 20010615

• The lac operator-repressor system is functional in the mouse.

  Genes & development 20010615

• Novel properties of the cyclin encoded by Human Herpesvirus 8 that facilitate exit from quiescence.

  Oncogene 20010607

• Enhanced inducible mGlu1alpha receptor expression in Chinese hamster ovary cells.

  Journal of neurochemistry 20010601

• Identification of a collagen-binding protein from Necator americanus by using a cDNA-expression phage display library.

  The Journal of parasitology 20010601

• degS (hhoB) is an essential Escherichia coli gene whose indispensable function is to provide sigma (E) activity.

  Molecular microbiology 20010601

• zur: a Zn(2+)-responsive regulatory element of Staphylococcus aureus.

  Microbiology (Reading, England) 20010501

• Stabilization of p53 by p14ARF without relocation of MDM2 to the nucleolus.

  Nature cell biology 20010501

• Enhanced expression of cytochrome P450s from lac-based plasmids using lactose as the inducer.

  Archives of biochemistry and biophysics 20010415

• Ectopic expression of MyD118/Gadd45/CR6 (Gadd45beta/alpha/gamma) sensitizes neoplastic cells to genotoxic stress-induced apoptosis.

  International journal of oncology 20010401

• Nucleotide sequence analysis of 5'-flanking region of salicylate hydroxylase gene, and identification and purification of a LysR-type regulator, SalR.

  European journal of biochemistry 20010401

• K-Ras-mediated increase in cyclooxygenase 2 mRNA stability involves activation of the protein kinase B1.

  Cancer research 20010315

• Isolation of Escherichia coli mRNA and comparison of expression using mRNA and total RNA on DNA microarrays.

  Analytical biochemistry 20010301

• Bacterial expression and characterization of starfish phospholipase A(2).

  Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology 20010301

• Improved osmotolerance of recombinant Escherichia coli by de novo glycine betaine biosynthesis.

  Applied microbiology and biotechnology 20010301

• Secretory production of Arthrobacter levan fructotransferase from recombinant Escherichia coli.

  FEMS microbiology letters 20010220

• Regulatory locus soxRS partially protects Escherichia coli against ozone.

  FEMS microbiology letters 20010220

• Metabolic engineering of the nonmevalonate isopentenyl diphosphate synthesis pathway in Escherichia coli enhances lycopene production.

  Biotechnology and bioengineering 20010220

• Chromosomal DNA demethylation specified by protein binding.

  EMBO reports 20010215

• Characterization of cytosolic sialidase from Chinese hamster ovary cells: part I: cloning and expression of soluble sialidase in Escherichia coli.

  Carbohydrate research 20010215

• Bacterial expression of an immunologically reactive PCV2 ORF2 fusion protein.

  Protein expression and purification 20010201

• Cloning, expression, and purification of histidine-tagged preS domains of hepatitis B virus.

  Protein expression and purification 20010201

• Alzheimer-associated neuronal thread protein-induced apoptosis and impaired mitochondrial function in human central nervous system-derived neuronal cells.

  Journal of neuropathology and experimental neurology 20010201

• Drug toxicity in E. coli cells expressing human topoisomerase I.

  Methods in molecular biology (Clifton, N.J.) 20010101

• Reduction of wobble-position GC bases in Corynebacteria genes and enhancement of PCR and heterologous expression.

  Journal of molecular microbiology and biotechnology 20010101

• Expression of kinesin in Escherichia coli.

  Methods in molecular biology (Clifton, N.J.) 20010101

• Transcription of the Escherichia coli dcw cluster: evidence for distal upstream transcripts being involved in the expression of the downstream ftsZ gene.

  Biochimie 20010101

• Reconstitution of protein-DNA complexes for crystallization.

  Methods in molecular biology (Clifton, N.J.) 20010101

• Purification of Moloney murine leukemia virus chromatin from infected cells by an affinity method.

  Journal of biomedical science 20010101

• Effects of in situ cobalt ion addition on the activity of a gfp-oph fusion protein: the fermentation kinetics.

  Biotechnology progress 20010101

• Confirming yeast two-hybrid protein interactions using in vitro glutathione-S-transferase pulldowns.

  Methods in molecular biology (Clifton, N.J.) 20010101

• Bacterial arylsulfate sulfotransferase as a reporter system.

  Microbiology and immunology 20010101

• Site-directed mutants of a soluble form of penicillin-binding protein 5 from Escherichia coli and their catalytic properties.

  The Journal of biological chemistry 19880205